Association of serum MicroRNA-145-5p levels with microvascular complications of type 1 Diabetes: The EURODIAB prospective complications study

Federica Barutta; Stefania Bellini; Simonetta Guarrera; Giuseppe Matullo; Casper Schalkwijk; Coen D. Stehouwer; Nish Chaturvedi; Sabita S. Soedamah-Muthu; Marilena Durazzo; Gabriella Gruden

doi:10.1016/j.diabres.2022.109987

Association of serum MicroRNA-145-5p levels with microvascular complications of type 1 Diabetes: The EURODIAB prospective complications study

Federica Barutta^*, Stefania Bellini, Simonetta Guarrera, Giuseppe Matullo, Casper Schalkwijk, Coen D. Stehouwer, Nish Chaturvedi, Sabita S. Soedamah-Muthu, Marilena Durazzo, Gabriella Gruden

^*Corresponding author for this work

Medical and Clinical Psychology

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Abstract

Aims
To investigate whether serum miR-145-5p levels were associated with micro-macrovascular chronic complications in patients with type 1 diabetes (DM1).

Methods
A nested case-control study from the EURODIAB Prospective Complications Study was performed. Cases (n = 289) had one or more complications of diabetes, whereas controls (n = 153) did not have any complication. We measured miR-145-5p levels by qPCR and investigated the association with diabetes complications.

Results
Mean miR-145-5p levels were significantly lower in cases with microangiopathy [2.12 (0.86–4.94)] compared to controls [3.15 (1.21–7.36), P < 0.05] even after adjustment for age, gender, and diabetes duration. In logistic regression analysis, miR-145-5p levels in the lowest tertile were associated with an over three-fold increased odds ratio (OR) of albuminuria [3.22 (1.17–8.81)], independently of both demographic and diabetes-related factors. In addition, mir145-5p levels in the lowest tertile were independently and inversely associated with arterial hypertension [1.96 (1.08–3.56)] and hypertension was the mediator of the relationship between miR-145-5p and albuminuria.

Conclusions
In this large cohort of DM1 patients, we found an inverse association between miR-145-5p and albuminuria that was mediated by systemic hypertension.

Original language	English
Article number	109987
Number of pages	8
Journal	Diabetes Research and Clinical Practice
Volume	190
DOIs	https://doi.org/10.1016/j.diabres.2022.109987
Publication status	Published - 2022

Keywords

Albuminuria
BIOMARKERS
CARDIOVASCULAR-DISEASE
CIRCULATING MICRORNAS
Cardiovascular diseases
Diabetic complications
Diabetic kidney disease
Diabetic nephropathy
EXPRESSION
Hypertension
MARKERS
MECHANISMS
MIRNAS
RETINOPATHY
SMOOTH-MUSCLE-CELLS
TARGETS
TGF-beta 1
Type 1 diabetes
microRNAs

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MCP Soedamah Muthu association of serum MicroRNA-145-5p DRaCP 2022Final published version, 0.98 MBLicence: CC BY-NC-ND

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Barutta, F., Bellini, S., Guarrera, S., Matullo, G., Schalkwijk, C., Stehouwer, C. D., Chaturvedi, N., Soedamah-Muthu, S. S., Durazzo, M., & Gruden, G. (2022). Association of serum MicroRNA-145-5p levels with microvascular complications of type 1 Diabetes: The EURODIAB prospective complications study. Diabetes Research and Clinical Practice, 190, Article 109987. https://doi.org/10.1016/j.diabres.2022.109987

@article{ab1c57549bd44fc19f7cd4463a566a74,

title = "Association of serum MicroRNA-145-5p levels with microvascular complications of type 1 Diabetes: The EURODIAB prospective complications study",

abstract = "AimsTo investigate whether serum miR-145-5p levels were associated with micro-macrovascular chronic complications in patients with type 1 diabetes (DM1).MethodsA nested case-control study from the EURODIAB Prospective Complications Study was performed. Cases (n = 289) had one or more complications of diabetes, whereas controls (n = 153) did not have any complication. We measured miR-145-5p levels by qPCR and investigated the association with diabetes complications.ResultsMean miR-145-5p levels were significantly lower in cases with microangiopathy [2.12 (0.86–4.94)] compared to controls [3.15 (1.21–7.36), P < 0.05] even after adjustment for age, gender, and diabetes duration. In logistic regression analysis, miR-145-5p levels in the lowest tertile were associated with an over three-fold increased odds ratio (OR) of albuminuria [3.22 (1.17–8.81)], independently of both demographic and diabetes-related factors. In addition, mir145-5p levels in the lowest tertile were independently and inversely associated with arterial hypertension [1.96 (1.08–3.56)] and hypertension was the mediator of the relationship between miR-145-5p and albuminuria.ConclusionsIn this large cohort of DM1 patients, we found an inverse association between miR-145-5p and albuminuria that was mediated by systemic hypertension.",

keywords = "Albuminuria, BIOMARKERS, CARDIOVASCULAR-DISEASE, CIRCULATING MICRORNAS, Cardiovascular diseases, Diabetic complications, Diabetic kidney disease, Diabetic nephropathy, EXPRESSION, Hypertension, MARKERS, MECHANISMS, MIRNAS, RETINOPATHY, SMOOTH-MUSCLE-CELLS, TARGETS, TGF-beta 1, Type 1 diabetes, microRNAs",

author = "Federica Barutta and Stefania Bellini and Simonetta Guarrera and Giuseppe Matullo and Casper Schalkwijk and Stehouwer, {Coen D.} and Nish Chaturvedi and Soedamah-Muthu, {Sabita S.} and Marilena Durazzo and Gabriella Gruden",

note = "This work was supported by the Cassa di Risparmio di Torino (CRT), the European Foundation for the Study of Diabetes (EFSD), Turin University (ex60%), Italian Ministry for Education, University and Research (Ministero dell'Istruzione, dell'Universit{\`a} e della Ricerca ‐ MIUR) under the program “Dipartimenti di Eccellenza 2018–2022” project D15D18000410001.",

year = "2022",

doi = "10.1016/j.diabres.2022.109987",

language = "English",

volume = "190",

journal = "Diabetes Research and Clinical Practice",

issn = "0168-8227",

publisher = "Elsevier Ireland Ltd",

}

Barutta, F, Bellini, S, Guarrera, S, Matullo, G, Schalkwijk, C, Stehouwer, CD, Chaturvedi, N, Soedamah-Muthu, SS, Durazzo, M & Gruden, G 2022, 'Association of serum MicroRNA-145-5p levels with microvascular complications of type 1 Diabetes: The EURODIAB prospective complications study', Diabetes Research and Clinical Practice, vol. 190, 109987. https://doi.org/10.1016/j.diabres.2022.109987

TY - JOUR

T1 - Association of serum MicroRNA-145-5p levels with microvascular complications of type 1 Diabetes

T2 - The EURODIAB prospective complications study

AU - Barutta, Federica

AU - Bellini, Stefania

AU - Guarrera, Simonetta

AU - Matullo, Giuseppe

AU - Schalkwijk, Casper

AU - Stehouwer, Coen D.

AU - Chaturvedi, Nish

AU - Soedamah-Muthu, Sabita S.

AU - Durazzo, Marilena

AU - Gruden, Gabriella

N1 - This work was supported by the Cassa di Risparmio di Torino (CRT), the European Foundation for the Study of Diabetes (EFSD), Turin University (ex60%), Italian Ministry for Education, University and Research (Ministero dell'Istruzione, dell'Università e della Ricerca ‐ MIUR) under the program “Dipartimenti di Eccellenza 2018–2022” project D15D18000410001.

PY - 2022

Y1 - 2022

N2 - AimsTo investigate whether serum miR-145-5p levels were associated with micro-macrovascular chronic complications in patients with type 1 diabetes (DM1).MethodsA nested case-control study from the EURODIAB Prospective Complications Study was performed. Cases (n = 289) had one or more complications of diabetes, whereas controls (n = 153) did not have any complication. We measured miR-145-5p levels by qPCR and investigated the association with diabetes complications.ResultsMean miR-145-5p levels were significantly lower in cases with microangiopathy [2.12 (0.86–4.94)] compared to controls [3.15 (1.21–7.36), P < 0.05] even after adjustment for age, gender, and diabetes duration. In logistic regression analysis, miR-145-5p levels in the lowest tertile were associated with an over three-fold increased odds ratio (OR) of albuminuria [3.22 (1.17–8.81)], independently of both demographic and diabetes-related factors. In addition, mir145-5p levels in the lowest tertile were independently and inversely associated with arterial hypertension [1.96 (1.08–3.56)] and hypertension was the mediator of the relationship between miR-145-5p and albuminuria.ConclusionsIn this large cohort of DM1 patients, we found an inverse association between miR-145-5p and albuminuria that was mediated by systemic hypertension.

AB - AimsTo investigate whether serum miR-145-5p levels were associated with micro-macrovascular chronic complications in patients with type 1 diabetes (DM1).MethodsA nested case-control study from the EURODIAB Prospective Complications Study was performed. Cases (n = 289) had one or more complications of diabetes, whereas controls (n = 153) did not have any complication. We measured miR-145-5p levels by qPCR and investigated the association with diabetes complications.ResultsMean miR-145-5p levels were significantly lower in cases with microangiopathy [2.12 (0.86–4.94)] compared to controls [3.15 (1.21–7.36), P < 0.05] even after adjustment for age, gender, and diabetes duration. In logistic regression analysis, miR-145-5p levels in the lowest tertile were associated with an over three-fold increased odds ratio (OR) of albuminuria [3.22 (1.17–8.81)], independently of both demographic and diabetes-related factors. In addition, mir145-5p levels in the lowest tertile were independently and inversely associated with arterial hypertension [1.96 (1.08–3.56)] and hypertension was the mediator of the relationship between miR-145-5p and albuminuria.ConclusionsIn this large cohort of DM1 patients, we found an inverse association between miR-145-5p and albuminuria that was mediated by systemic hypertension.

KW - Albuminuria

KW - BIOMARKERS

KW - CARDIOVASCULAR-DISEASE

KW - CIRCULATING MICRORNAS

KW - Cardiovascular diseases

KW - Diabetic complications

KW - Diabetic kidney disease

KW - Diabetic nephropathy

KW - EXPRESSION

KW - Hypertension

KW - MARKERS

KW - MECHANISMS

KW - MIRNAS

KW - RETINOPATHY

KW - SMOOTH-MUSCLE-CELLS

KW - TARGETS

KW - TGF-beta 1

KW - Type 1 diabetes

KW - microRNAs

UR - http://www.scopus.com/inward/record.url?scp=85134301253&partnerID=8YFLogxK

U2 - 10.1016/j.diabres.2022.109987

DO - 10.1016/j.diabres.2022.109987

M3 - Article

SN - 0168-8227

VL - 190

JO - Diabetes Research and Clinical Practice

JF - Diabetes Research and Clinical Practice

M1 - 109987

ER -

Association of serum MicroRNA-145-5p levels with microvascular complications of type 1 Diabetes: The EURODIAB prospective complications study

Abstract

Keywords

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