The ability to update responding to threat cues is an important adaptive ability. Recently, Morriss and colleagues (2019) demonstrated that participants scoring high in Intolerance of Uncertainty (IU) were more capable of threat reversal. The current report aimed to conceptually replicate these results of Morriss et al. (2019) in an independent sample using a comparable paradigm (n = 102). Following a threat conditioning phase, participants were told that cues associated with threat and safety from electric shock would reverse. Responding was measured with skin conductance and fear potentiated startle. We failed to replicate the results of Morriss et al., (2019). Instead, we found that individuals with lower IU, relative to higher IU, who received contingency instructions prior to acquisition were more capable of threat reversal, indexed via skin conductance response. These results suggest that IU and contingency instructions differentially modulate the course of threat reversal.