MMP-14 and CD44 in Epithelial-to-Mesenchymal Transition (EMT) in ovarian cancer

M.C. Vos, E. Hollemans, N.P.M. Ezendam, H. Feijen, D. Boll, B. Pijlman, H. van der Putten, P. Klinkhamer, T.H. Kuppevelt, A.A. van de Wurff, L.F. Massuger

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Abstract

Background
To investigate the expression of MMP-14 and CD44 as well as epithelial-to-mesenchymal transition(EMT)-like changes in ovarian cancer and to determine correlations with clinical outcome.
Methods
In 97 patients with ovarian cancer, MMP-14 and CD44 expression as determined by immunohistochemistry was investigated in relation to EMT-like changes. To determine this, immunohistochemical staining of E-cadherin and vimentin was performed.
Results
Patients with expression of both MMP-14 and CD44 in their tumors had a poor prognosis despite complete debulking. Serous histology in advanced-stage tumors (FIGO IIB-IV) correlated with CD44 (rho .286, p < 0.01). Also, CD44 correlated with percentage vimentin expression (rho .217, p < 0.05).In logistic regression analysis with complete debulking as the outcome parameter, CD44 expression was found to be significant (OR 3,571 (95 % Confidence Interval 1,112–11,468) p = 0.032), though this was not the case for MMP-14 and EMT parameters.
Conclusion
The subgroup of patients with double expression of MMP-14 and CD44 had a poor prognosis despite complete debulking. Serous subtype in advanced-stage patients and CD44 expression were found to be correlated with vimentin expression, and CD44 expression was found to be significantly correlated with complete debulking. However, a significant correlation between EMT and clinical parameters was not found.KeywordsMMP-14, CD44, Epithelial-to-mesenchymal transition, Ovarian cancer
Original languageEnglish
Article number53
JournalJournal of Ovarian Research
Volume9
Issue number1
DOIs
Publication statusPublished - 2 Sep 2016

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Matrix Metalloproteinases
Ovarian Neoplasms
Vimentin
Neoplasms
Logistic Models
Confidence Intervals

Cite this

Vos, M.C. ; Hollemans, E. ; Ezendam, N.P.M. ; Feijen, H. ; Boll, D. ; Pijlman, B. ; van der Putten, H. ; Klinkhamer, P. ; Kuppevelt, T.H. ; van de Wurff, A.A. ; Massuger, L.F. / MMP-14 and CD44 in Epithelial-to-Mesenchymal Transition (EMT) in ovarian cancer. In: Journal of Ovarian Research. 2016 ; Vol. 9, No. 1.
@article{f3291fe189eb4034b72ae8dd89e2a86e,
title = "MMP-14 and CD44 in Epithelial-to-Mesenchymal Transition (EMT) in ovarian cancer",
abstract = "BackgroundTo investigate the expression of MMP-14 and CD44 as well as epithelial-to-mesenchymal transition(EMT)-like changes in ovarian cancer and to determine correlations with clinical outcome.MethodsIn 97 patients with ovarian cancer, MMP-14 and CD44 expression as determined by immunohistochemistry was investigated in relation to EMT-like changes. To determine this, immunohistochemical staining of E-cadherin and vimentin was performed.ResultsPatients with expression of both MMP-14 and CD44 in their tumors had a poor prognosis despite complete debulking. Serous histology in advanced-stage tumors (FIGO IIB-IV) correlated with CD44 (rho .286, p < 0.01). Also, CD44 correlated with percentage vimentin expression (rho .217, p < 0.05).In logistic regression analysis with complete debulking as the outcome parameter, CD44 expression was found to be significant (OR 3,571 (95 {\%} Confidence Interval 1,112–11,468) p = 0.032), though this was not the case for MMP-14 and EMT parameters.ConclusionThe subgroup of patients with double expression of MMP-14 and CD44 had a poor prognosis despite complete debulking. Serous subtype in advanced-stage patients and CD44 expression were found to be correlated with vimentin expression, and CD44 expression was found to be significantly correlated with complete debulking. However, a significant correlation between EMT and clinical parameters was not found.KeywordsMMP-14, CD44, Epithelial-to-mesenchymal transition, Ovarian cancer",
author = "M.C. Vos and E. Hollemans and N.P.M. Ezendam and H. Feijen and D. Boll and B. Pijlman and {van der Putten}, H. and P. Klinkhamer and T.H. Kuppevelt and {van de Wurff}, A.A. and L.F. Massuger",
year = "2016",
month = "9",
day = "2",
doi = "10.1186/s13048-016-0262-7",
language = "English",
volume = "9",
journal = "Journal of Ovarian Research",
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Vos, MC, Hollemans, E, Ezendam, NPM, Feijen, H, Boll, D, Pijlman, B, van der Putten, H, Klinkhamer, P, Kuppevelt, TH, van de Wurff, AA & Massuger, LF 2016, 'MMP-14 and CD44 in Epithelial-to-Mesenchymal Transition (EMT) in ovarian cancer', Journal of Ovarian Research, vol. 9, no. 1, 53. https://doi.org/10.1186/s13048-016-0262-7

MMP-14 and CD44 in Epithelial-to-Mesenchymal Transition (EMT) in ovarian cancer. / Vos, M.C.; Hollemans, E.; Ezendam, N.P.M.; Feijen, H.; Boll, D.; Pijlman, B.; van der Putten, H.; Klinkhamer, P.; Kuppevelt, T.H.; van de Wurff, A.A.; Massuger, L.F.

In: Journal of Ovarian Research, Vol. 9, No. 1, 53, 02.09.2016.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - MMP-14 and CD44 in Epithelial-to-Mesenchymal Transition (EMT) in ovarian cancer

AU - Vos, M.C.

AU - Hollemans, E.

AU - Ezendam, N.P.M.

AU - Feijen, H.

AU - Boll, D.

AU - Pijlman, B.

AU - van der Putten, H.

AU - Klinkhamer, P.

AU - Kuppevelt, T.H.

AU - van de Wurff, A.A.

AU - Massuger, L.F.

PY - 2016/9/2

Y1 - 2016/9/2

N2 - BackgroundTo investigate the expression of MMP-14 and CD44 as well as epithelial-to-mesenchymal transition(EMT)-like changes in ovarian cancer and to determine correlations with clinical outcome.MethodsIn 97 patients with ovarian cancer, MMP-14 and CD44 expression as determined by immunohistochemistry was investigated in relation to EMT-like changes. To determine this, immunohistochemical staining of E-cadherin and vimentin was performed.ResultsPatients with expression of both MMP-14 and CD44 in their tumors had a poor prognosis despite complete debulking. Serous histology in advanced-stage tumors (FIGO IIB-IV) correlated with CD44 (rho .286, p < 0.01). Also, CD44 correlated with percentage vimentin expression (rho .217, p < 0.05).In logistic regression analysis with complete debulking as the outcome parameter, CD44 expression was found to be significant (OR 3,571 (95 % Confidence Interval 1,112–11,468) p = 0.032), though this was not the case for MMP-14 and EMT parameters.ConclusionThe subgroup of patients with double expression of MMP-14 and CD44 had a poor prognosis despite complete debulking. Serous subtype in advanced-stage patients and CD44 expression were found to be correlated with vimentin expression, and CD44 expression was found to be significantly correlated with complete debulking. However, a significant correlation between EMT and clinical parameters was not found.KeywordsMMP-14, CD44, Epithelial-to-mesenchymal transition, Ovarian cancer

AB - BackgroundTo investigate the expression of MMP-14 and CD44 as well as epithelial-to-mesenchymal transition(EMT)-like changes in ovarian cancer and to determine correlations with clinical outcome.MethodsIn 97 patients with ovarian cancer, MMP-14 and CD44 expression as determined by immunohistochemistry was investigated in relation to EMT-like changes. To determine this, immunohistochemical staining of E-cadherin and vimentin was performed.ResultsPatients with expression of both MMP-14 and CD44 in their tumors had a poor prognosis despite complete debulking. Serous histology in advanced-stage tumors (FIGO IIB-IV) correlated with CD44 (rho .286, p < 0.01). Also, CD44 correlated with percentage vimentin expression (rho .217, p < 0.05).In logistic regression analysis with complete debulking as the outcome parameter, CD44 expression was found to be significant (OR 3,571 (95 % Confidence Interval 1,112–11,468) p = 0.032), though this was not the case for MMP-14 and EMT parameters.ConclusionThe subgroup of patients with double expression of MMP-14 and CD44 had a poor prognosis despite complete debulking. Serous subtype in advanced-stage patients and CD44 expression were found to be correlated with vimentin expression, and CD44 expression was found to be significantly correlated with complete debulking. However, a significant correlation between EMT and clinical parameters was not found.KeywordsMMP-14, CD44, Epithelial-to-mesenchymal transition, Ovarian cancer

U2 - 10.1186/s13048-016-0262-7

DO - 10.1186/s13048-016-0262-7

M3 - Article

VL - 9

JO - Journal of Ovarian Research

JF - Journal of Ovarian Research

SN - 1757-2215

IS - 1

M1 - 53

ER -