MMP-14 and CD44 in Epithelial-to-Mesenchymal Transition (EMT) in ovarian cancer

M.C. Vos; E. Hollemans; N.P.M. Ezendam; H. Feijen; D. Boll; B. Pijlman; H. van der Putten; P. Klinkhamer; T.H. Kuppevelt; A.A. van de Wurff; L.F. Massuger

doi:10.1186/s13048-016-0262-7

MMP-14 and CD44 in Epithelial-to-Mesenchymal Transition (EMT) in ovarian cancer

M.C. Vos
, E. Hollemans
, N.P.M. Ezendam
, H. Feijen
, D. Boll
, B. Pijlman
, H. van der Putten
, P. Klinkhamer
, T.H. Kuppevelt
, A.A. van de Wurff
, L.F. Massuger

Medical and Clinical Psychology

Research output: Contribution to journal › Article › Scientific › peer-review

30 Citations (Scopus)

118 Downloads (Pure)

Abstract

Background
To investigate the expression of MMP-14 and CD44 as well as epithelial-to-mesenchymal transition(EMT)-like changes in ovarian cancer and to determine correlations with clinical outcome.
Methods
In 97 patients with ovarian cancer, MMP-14 and CD44 expression as determined by immunohistochemistry was investigated in relation to EMT-like changes. To determine this, immunohistochemical staining of E-cadherin and vimentin was performed.
Results
Patients with expression of both MMP-14 and CD44 in their tumors had a poor prognosis despite complete debulking. Serous histology in advanced-stage tumors (FIGO IIB-IV) correlated with CD44 (rho .286, p < 0.01). Also, CD44 correlated with percentage vimentin expression (rho .217, p < 0.05).In logistic regression analysis with complete debulking as the outcome parameter, CD44 expression was found to be significant (OR 3,571 (95 % Confidence Interval 1,112–11,468) p = 0.032), though this was not the case for MMP-14 and EMT parameters.
Conclusion
The subgroup of patients with double expression of MMP-14 and CD44 had a poor prognosis despite complete debulking. Serous subtype in advanced-stage patients and CD44 expression were found to be correlated with vimentin expression, and CD44 expression was found to be significantly correlated with complete debulking. However, a significant correlation between EMT and clinical parameters was not found.KeywordsMMP-14, CD44, Epithelial-to-mesenchymal transition, Ovarian cancer

Original language	English
Article number	53
Journal	Journal of Ovarian Research
Volume	9
Issue number	1
DOIs	https://doi.org/10.1186/s13048-016-0262-7
Publication status	Published - 2 Sept 2016

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Access to Document

10.1186/s13048-016-0262-7

MCP Ezendam MMP-14 JoOR open access 2016Final published version, 1.41 MB

Cite this

@article{f3291fe189eb4034b72ae8dd89e2a86e,

title = "MMP-14 and CD44 in Epithelial-to-Mesenchymal Transition (EMT) in ovarian cancer",

abstract = "Background To investigate the expression of MMP-14 and CD44 as well as epithelial-to-mesenchymal transition(EMT)-like changes in ovarian cancer and to determine correlations with clinical outcome. Methods In 97 patients with ovarian cancer, MMP-14 and CD44 expression as determined by immunohistochemistry was investigated in relation to EMT-like changes. To determine this, immunohistochemical staining of E-cadherin and vimentin was performed. Results Patients with expression of both MMP-14 and CD44 in their tumors had a poor prognosis despite complete debulking. Serous histology in advanced-stage tumors (FIGO IIB-IV) correlated with CD44 (rho .286, p < 0.01). Also, CD44 correlated with percentage vimentin expression (rho .217, p < 0.05).In logistic regression analysis with complete debulking as the outcome parameter, CD44 expression was found to be significant (OR 3,571 (95 \% Confidence Interval 1,112–11,468) p = 0.032), though this was not the case for MMP-14 and EMT parameters. Conclusion The subgroup of patients with double expression of MMP-14 and CD44 had a poor prognosis despite complete debulking. Serous subtype in advanced-stage patients and CD44 expression were found to be correlated with vimentin expression, and CD44 expression was found to be significantly correlated with complete debulking. However, a significant correlation between EMT and clinical parameters was not found.KeywordsMMP-14, CD44, Epithelial-to-mesenchymal transition, Ovarian cancer ",

author = "M.C. Vos and E. Hollemans and N.P.M. Ezendam and H. Feijen and D. Boll and B. Pijlman and \{van der Putten\}, H. and P. Klinkhamer and T.H. Kuppevelt and \{van de Wurff\}, A.A. and L.F. Massuger",

year = "2016",

month = sep,

day = "2",

doi = "10.1186/s13048-016-0262-7",

language = "English",

volume = "9",

journal = "Journal of Ovarian Research",

publisher = "BioMed Central",

number = "1",

}

TY - JOUR

T1 - MMP-14 and CD44 in Epithelial-to-Mesenchymal Transition (EMT) in ovarian cancer

AU - Vos, M.C.

AU - Hollemans, E.

AU - Ezendam, N.P.M.

AU - Feijen, H.

AU - Boll, D.

AU - Pijlman, B.

AU - van der Putten, H.

AU - Klinkhamer, P.

AU - Kuppevelt, T.H.

AU - van de Wurff, A.A.

AU - Massuger, L.F.

PY - 2016/9/2

Y1 - 2016/9/2

N2 - Background To investigate the expression of MMP-14 and CD44 as well as epithelial-to-mesenchymal transition(EMT)-like changes in ovarian cancer and to determine correlations with clinical outcome. Methods In 97 patients with ovarian cancer, MMP-14 and CD44 expression as determined by immunohistochemistry was investigated in relation to EMT-like changes. To determine this, immunohistochemical staining of E-cadherin and vimentin was performed. Results Patients with expression of both MMP-14 and CD44 in their tumors had a poor prognosis despite complete debulking. Serous histology in advanced-stage tumors (FIGO IIB-IV) correlated with CD44 (rho .286, p < 0.01). Also, CD44 correlated with percentage vimentin expression (rho .217, p < 0.05).In logistic regression analysis with complete debulking as the outcome parameter, CD44 expression was found to be significant (OR 3,571 (95 % Confidence Interval 1,112–11,468) p = 0.032), though this was not the case for MMP-14 and EMT parameters. Conclusion The subgroup of patients with double expression of MMP-14 and CD44 had a poor prognosis despite complete debulking. Serous subtype in advanced-stage patients and CD44 expression were found to be correlated with vimentin expression, and CD44 expression was found to be significantly correlated with complete debulking. However, a significant correlation between EMT and clinical parameters was not found.KeywordsMMP-14, CD44, Epithelial-to-mesenchymal transition, Ovarian cancer

AB - Background To investigate the expression of MMP-14 and CD44 as well as epithelial-to-mesenchymal transition(EMT)-like changes in ovarian cancer and to determine correlations with clinical outcome. Methods In 97 patients with ovarian cancer, MMP-14 and CD44 expression as determined by immunohistochemistry was investigated in relation to EMT-like changes. To determine this, immunohistochemical staining of E-cadherin and vimentin was performed. Results Patients with expression of both MMP-14 and CD44 in their tumors had a poor prognosis despite complete debulking. Serous histology in advanced-stage tumors (FIGO IIB-IV) correlated with CD44 (rho .286, p < 0.01). Also, CD44 correlated with percentage vimentin expression (rho .217, p < 0.05).In logistic regression analysis with complete debulking as the outcome parameter, CD44 expression was found to be significant (OR 3,571 (95 % Confidence Interval 1,112–11,468) p = 0.032), though this was not the case for MMP-14 and EMT parameters. Conclusion The subgroup of patients with double expression of MMP-14 and CD44 had a poor prognosis despite complete debulking. Serous subtype in advanced-stage patients and CD44 expression were found to be correlated with vimentin expression, and CD44 expression was found to be significantly correlated with complete debulking. However, a significant correlation between EMT and clinical parameters was not found.KeywordsMMP-14, CD44, Epithelial-to-mesenchymal transition, Ovarian cancer

U2 - 10.1186/s13048-016-0262-7

DO - 10.1186/s13048-016-0262-7

M3 - Article

VL - 9

JO - Journal of Ovarian Research

JF - Journal of Ovarian Research

IS - 1

M1 - 53

ER -

MMP-14 and CD44 in Epithelial-to-Mesenchymal Transition (EMT) in ovarian cancer

Abstract

UN SDGs

Access to Document

Fingerprint

Cite this